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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Immunol. 2019 Aug 26;203(7):1989–1998. doi: 10.4049/jimmunol.1900128

Fig 5. Bach2 functionally cooperates with BATF for IL-9 production in Th17 cells.

Fig 5.

Naive cells were cultured under Th17 condition (A-C). (A) MSCV-Thy1.1 control or Bach2-Thy1.1 expressing retrovirus was transduced on day 1. Cytokine production was detected after 5 hours PMA/ionomycin stimulation on day 5. (B-C) MIEG-GFP and MSCV-Thy1.1 control (open box) or BATF-GFP and Bach2-Thy1.1 (closed box) expressing retrovirus were co-transduced on day 1. Cytokine production was detected after 5 hours PMA/ionomycin stimulation on day 5. Contour plots were gated on live CD4+ transduced cells and results are displayed as histograms (B) and bar graphs (C) of gated populations. Data are mean ± SEM of 3 mice per experiment and representative of three independent experiments. A two tailed Student’s t-test was used for pairwise comparisons. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.