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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jul 18;39(10):2028–2037. doi: 10.1161/ATVBAHA.119.312439

Figure 1.

Figure 1

ARC1779 inhibits refrigeration-mediated VWF binding. A, Platelet refrigeration strategy. Human or murine WT PRPs were refrigerated with or without ARC1779 for 48 hours. Refrigerated platelets and fresh platelets were then warmed to 37°C, and VWF binding was analyzed by flow cytometry. B and C, Flow cytometry histogram of VWF binding to human platelets (B) and mouse platelets (C). The histogram of the negative control is filled by gray area. The dotted black line is fresh platelets. The dashed black line is refrigerated platelets treated with ARC1779, and the solid black line is untreated refrigerated platelets. D, Quantification of VWF binding to human platelets (n=3, left) and mouse platelets (n=3, right). Each VWF binding was quantified by mean fluorescence intensity of the histogram, subtracted by that of negative control, and normalized with that of fresh platelets as 1. Statistical analysis was performed by 2-way ANOVA. All data are shown as mean±SD. *, P < 0.05; and ***, P < 0.001.