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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jul 18;39(10):2028–2037. doi: 10.1161/ATVBAHA.119.312439

Figure 4.

Figure 4

Antidote treatment increases the hemostatic function of ARC1779-treated refrigerated platelets. A-B, Tail bleeding assay two hours after transfusion. IL4R-IbαTg mice were transfused with PBS, fresh, refrigerated, and refrigerated with ARC1779 WT platelets. Two hours after transfusion, 2 mm segment of the mouse tail was cut off, and the tail vein was put into warmed saline. Bleeding time (A) and the blood loss (B) then was recorded. C-D, Tail bleeding assay fifteen minutes after transfusion. IL4R-IbαTg mice were transfused with fresh, refrigerated, ARC1779-treated refrigerated platelets, and ARC1779/antidote-treated refrigerated platelets. Tail bleeding time assays were performed at 15 minutes after transfusion, and bleeding time (C) and blood loss (D) were measured. Statistical analysis was performed by 2-way ANOVA. All data are presented as mean±SD (n=3–7). n.s., not significant; *, P < 0.05; and **, P < 0.01.