Figure 9. Inducible Lpl deletion dramatically decreases both resident and recruited peritoneal macrophages content upon zymosan stimuli.

Lean littermate controls (Lpl^fl/fl) and iLpl^−/− (Lpl^fl/fl;β-actin-MerCreMer) mice were studied for both resident peritoneal macrophages (F4/80^highMHCII^low) and recruited peritoneal macrophages (F4/80^lowMHCII^high) under zymosan-induced peritonitis. (A) Experimental design: Zymosan (100 μg/mouse) was i.p injected into 12-week-old lean Lpl^fl/fl and Mac-Lpl^−/−mice on day 0 to induce monocyte/macrophage recruitment to peritoneal cavity. All mice were sacrificed on day 3 for peritoneal macrophage analysis using flow cytometry. (B) Representative flow cytometry plots for peritoneal macrophages subpopulations. (C) Flow cytometry analysis of resident peritoneal macrophages. (D) Flow cytometry analysis of recruited peritoneal macrophages. N=3-5/group. *p<0.05. Results are represented as median with 25^th and 75^th percentiles, capped bars at 10^th and 90^th percentile and compared using Two-Way ANOVA with Sidak’s multiple comparison test.