TABLE I.
Antisera Against | Marks | Company, Catalog No., RRID No. | Host, Dilution | Secondary Antibody | Antisera Against |
---|---|---|---|---|---|
Acetylated tubulin | Nerve fibers, ciliated cells | Sigma, T7451, AB_609894 | Mouse, 1:5000 | Alexa 647 | Donkey anti-mouse |
α-gustducin (GNAT3) | G-protein subunit in type II taste cells and solitary chemosensory cells | Aviva System Biology Corp., OAEB00418, AB_10882823 | Goat, 1:500 | Alexa 488; Alexa 647 | Donkey anti-goat |
Carbonic anhydrase 4 (CA4) | Type III cells | R&D Systems, AF2414, AB_2070332 | Goat, 1:500 | Alexa 488 | Donkey anti-goat |
PGP9.5 (UCHL-1) | Neurons and nerve fibers; subpopulation of type III cells | Novus Biologicals, NB110–58872SS AB_877619 | Chicken, 1:2000 | Alexa 488 | Donkey anti-chicken |
P2X3 | ATP receptor on taste nerves and pain fibers | Alomone Labs, APR-016, AB_2341047 | Rabbit, 1:1000 | Alexa 568 | Donkey anti-rabbit |
P2X3 | ATP receptor on taste nerves and pain fibers | Neuromics, GP10108, AB_2283325 | Guinea pig, 1:500 | Alexa 488 | Donkey anti-guinea pig |
PGP9.5 | Neurons and nerve fibers; subpopulation of type III cells | ABD Serotec, 7863–0504, AB_2210505 | Rabbit, 1:500 | Alexa 568 | Donkey anti-rabbit |
PLCβ2 | Transduction component in type II taste cells | Santa Cruz Biotechnology, SC-206, AB_632197 | Rabbit, 1:500 | Alexa 568 | Donkey anti-rabbit |
SNAP25 | Neurons and nerve fibers; subpopulation of type III cells | Sigma, S9684, AB_261576 | Rabbit, 1:500 | Alexa 568 | Donkey anti-rabbit |
ATP = adenosine triphosphate; RRID = research resource identifier.