TABLE II.

Antibodies for Mouse Tissue

Antisera Against	Marks	Company, Catalog No., RRID No.	Host, Dilution	Secondary Antibody	Antisera Against
Acetylated tubulin*	Nerve fibers, ciliated cells	Sigma, T7451, AB_609894	Mouse, 1:5000	Alexa 647	Donkey anti-mouse
α-gustducin (GNAT3)	G-protein subunit in type II taste cells and solitary chemosensory cells	Aviva System Biology Corp., OAEB00418, AB_10882823	Goat, 1:500	Alexa 488; Alexa 647	Donkey anti-goat
Carbonic anhydrase 4 (CA4)	Type III cells	R&D System, AF2414, AB_2070332	Goat, 1:1000	Alexa 488	Donkey anti-goat
P2X3	ATP receptor on taste nerves and pain fibers	Neuromics, GP10108, AB_2283325	Guinea pig, 1:500	Alexa 488	Donkey anti-guinea pig
PGP9.5 (UCHL-1)	Neurons and nerve fibers, subpopulation of type III cells	Novus Biologicals, NB110–58872SS, AB_877619	Chicken, 1:2000	Alexa 488	Donkey anti-chicken
PLCβ2	Transduction component in type II taste cells	Santa Cruz Biotechnology, SC-206, AB_632197	Rabbit, 1:500	Alexa 568	Donkey anti-rabbit
SNAP25	Neurons and nerve fibers, subpopulation of type III cells	GeneTex, GTX89577, AB_10724125	Goat, 1:1000	Alexa 488	Donkey anti-goat
Substance P	Nerve fibers	Accurate Chemicals, YMC1021, AB_2333091	Rat, 1:1000	Alexa 488	Donkey anti-rat

^*AffiniPure Fab Fragment Donkey anti-mouse immunoglobulin G (H + L), Jackson ImmunoResearch, 715–007-003, used for blocking of endogenous immunoglobulins on mouse tissue

ATP = adenosine triphosphate; RRID = research resource identifier.