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. 2019 Sep 25;9:13906. doi: 10.1038/s41598-019-50471-y

Figure 2.

Figure 2

PD protects chondrocytes against IL-1β-induced apoptosis and ROS generation. In vitro pretreated with PD and 3-MA, representative ROS (A,B) and the percentage of live cells (G,H) was measured and quantitative counted by flow cytometry. (CF) qRT-PCR was performed to analyze the apoptosis-related gene expression levels (Bcl-2, BAX, caspase-3, and the ratio of Bcl-2/BAX) in vitro. Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. Values are the means ± SD (n = 5).