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. 2019 Sep 25;9:13906. doi: 10.1038/s41598-019-50471-y

Figure 3.

Figure 3

PD restores chondrocyte function in vitro. (AD) Western blot of Col I, IL-6, and TNF-α in different groups were detected respectively and the semi-quantitative analysis of Col I/GAPDH, IL-6/GAPDH, and TNF-α/GAPDH are shown. (EK) qRT-PCR was performed to determine the expression levels of (EH) Col I, Col II, SOX9 and ACAN and (IK) osteoarthritis relative gene (IL-6, TNF-α and MMP-13). Values are the means ± SD, n = 5. Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. (L) Immunohistochemical staining images revealed the presence of Col I, Col II, IL-6, and TNF-α. Original magnification × 200 (scale bar, 300 μm).