Fig. 3.

RNA sensing and IFNAR signaling control ZIKV replication and dissemination. Groups of DMPA-treated female mice were i.vag. inoculated with 2 × 10⁴ FFU of ZIKV (PRVABC59). FFU equivalent of ZIKV detected by qRT-PCR from a LFRT (TKO n = 5, Mavs^−/− Tlr3^−/− n = 7, Mavs^−/− Tlr7^−/− n = 8, Tlr3^−/− n = 9, and Tlr3^−/− Tlr7^−/− n = 10, Mavs^−/− n = 11, Ifnar1^−/− n = 14, WT and Tlr7^−/− n = 16 mice), b UFRT (Mavs^−/− Tlr7^−/− and Mavs^−/− Tlr3^−/− n = 4, Tlr3^−/−, Tlr7^−/− Tlr3^−/− and TKO n = 5, Ifnar1^−/− n = 6, Tlr7^−/− n = 8, Mavs^−/− n = 9, and WT n = 12 mice), c iLN (Mavs^−/− Tlr3^−/− n = 4, Tlr3^−/−, Tlr7^−/−, Tlr7^−/− Tlr3^−/− and TKO n = 5, WT, Mavs^−/− and Ifnar1^−/− n = 6, Mavs^−/− Tlr7^−/− n = 8 mice), and d spleen (Mavs^−/− Tlr3^−/− n = 4, Tlr3^−/−, Tlr7^−/−, Tlr7^−/− Tlr3^−/− and TKO n = 5, WT, Mavs^−/− and Ifnar1^−/− n = 6, Mavs^−/− Tlr7^−/− n = 8 mice) of i.vag. infected mice at day 6 post-infection. Data pulled from two independent experiments; mean ± SEM *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Kruskal–Wallis test with Tukey’s multiple comparison test. Each dot represents value from one mouse. Source data for Fig. 3a–d are provided as a Source Data file