Fig. 4.

Low expression of RNA sensors in vaginal mucosa of mice and macaques. Tissues were collected from C57BL/6N female mice one week after DMPA treatment (upper panels), or from uninfected female macaques without any DMPA treatment (lower panels). Using qRT-PCR, levels of mRNA of indicated genes in all tissues were detected, normalized to GAPDH and expressed as fold-change over iLN samples. a Expression of RNA sensors. b Expression of TLR3 co-receptor. Mouse LFRT and UFRT n = 12, mouse iLN n = 13, macaque vag1, vag3, uterus and iLN n = 6, macaque cervix and ovary n = 5. Data represent mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test. Each dot represents value from one animal. Vag, vagina (Vag 1 and Vag 3 represent two different regions of the vaginal tissue from the same animal); iLN, iliac lymph node; LFRT, lower female reproductive tract; UFRT, upper female reproductive tract. Source data for Fig. 4a–b are provided as a Source Data file