Fig. 5.

Expression of RNA sensors in DMPA-treated versus naturally cycling mice. Tissues were collected from C57BL/6N female mice one week after DMPA treatment (1.0 mg/mouse), or immediately after determining the estrus or diestrus cycle by visual monitoring of inflammation followed by microscopic confirmation of cells in the vaginal lavage. Using qRT-PCR, levels of mRNA of indicated genes in all tissues were detected, normalized to GAPDH and expressed as fold-change over iLN samples within each group. Data represent mean ± SEM; n = 6 mice per group, except n = 5 in LFRT diestrus; ***p < 0.001; two-way ANOVA with Dunnett’s multiple comparison test. Each dot represents value from one animal. iLN, iliac lymph node; LFRT, lower female reproductive tract; UFRT, upper female reproductive tract. Source data for Fig. 5 are provided as a Source Data file