Fig. 7.

IFNAR signaling is required to limit viral dissemination from LFRT to other tissues. Female mice or macaques were i.vag. inoculated with ZIKV, and tissues were collected at day 4 or 8 post-infection. Indicated genes in total RNA were detected using qRT-PCR from (a) C57BL/6N WT (UI n = 6–7, D4 n = 7 except LFRT, D4 LFRT n = 4, D8 LFRT and iLN n = 8, and D8 UFRT n = 11 mice), (b) Tlr3^−/− Tlr7^−/− Mavs^−/− TKO mice (UI n = 5, D4 n = 5, D8 n = 6 animals), (c) Ifnar1^−/− mice (UI n = 6, D4 LFRT n = 6, D4 UFRT and iLN n = 4, and D8 n = 4 animals), or (d) macaques (UI sample was pooled from samples shown in Fig. 4 for reference, one animal each for D4 and D8), normalized to GAPDH, and expressed as fold-changes over iLN samples from naïve animals. (e) ZIKV RNA was detected by qRT-PCR in total RNA from the indicated tissues of WT (D4 n = 7 and D8 n = 5), TKO (D4 n = 5 and D8 n = 6), and Ifnar1^−/− (D4 n = 4 and D8 n = 4) mice. UI WT mice (n = 4) were used to determine the background level of the qRT-PCR assay. All female mice were treated with 1.0 mg DMPA followed by i.vag. infection a week later. Previously described macaques were treated with 30 mg of DMPA 4 weeks prior and on the day of infection²⁰. The historical ZIKV RNA levels in the macaque tissues have been re-plotted. Data represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; two-way ANOVA with Tukey’s multiple comparison test. Each dot represent value from one animal and each time-point represents data from a separate group of animals. Vag, vagina (macaque equivalent of LFRT in mice); iLN, iliac lymph node; LFRT, lower female reproductive tract; UFRT, upper female reproductive tract; UI, uninfected. Source data for Fig. 6a–d are provided as a Source Data file