Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 25;9:13891. doi: 10.1038/s41598-019-50210-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Functional validation of miR-27a/b-3p effects on PPARG, SCAMP3 and ABCA1 in human adipocytes. (a) miR-27a-3p and miR-27b-3p were overexpressed in in vitro differentiated in hASCs and their expression was assessed by RT-qPCR. Results are based on three biological/independent experiments. Expression of genes was normalized to the reference gene SNORD68. (b) miR-27a-3p and miR-27b-3p were overexpressed in in vitro differentiated in hASCs and expression of PPARG, SCAMP3 and ABCA1 was assessed by RT-qPCR. Results are based on three biological/independent experiments. Expression of genes was normalized to the reference gene LRP10. (c,d) miR-27a-3p and miR-27b-3p were overexpressed in in vitro differentiated in hASCs, cells were lyzed to collect the total protein and thereafter proteins were analyzed by Western blot. Results are based on four biological/independent experiments. Expression of SCAMP3 was normalized to the total protein amount. (e) Mimics of miR-27a-3p and miR-27b-3p were transfected together with 3′UTR reporter constructs for SCAMP3, ABCA1 or empty reporter vector in 3T3-L1 cells and changes of luciferase activity was measured in cell lysates. Results are based on two or three biological/independent experiments. Results were analyzed using t-test and presented in fold change ± SD relative to negative control (Neg C). ***P < 0.005, *P < 0.05.