Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 25;9:13891. doi: 10.1038/s41598-019-50210-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Impact of SCAMP3 on human adipogenesis. (a) Expression of PPARG was knocked down using siRNA in hASCs 24 h before induction of differentiation and until days 2, 6 and 9 of differentiation, upon which the expression of PPARG and SCAMP3 was monitored. (b) Expression of SCAMP3 was knocked down using siRNA in hASCs 24 h before induction of differentiation until days 2, 6 and 9 of differentiation, upon which the expression of SCAMP3 and PPARG was monitored. (c) Expression of SCAMP3 and PPARG was knocked down using siRNA in hASCs 24 h before induction of differentiation until day 9 of differentiation when accumulation of neutral lipids and number of cells was evaluated. (d) Expression of SCAMP3 was knocked down using siRNA in hASCs 24 h before induction of differentiation until days 2, 6 and 9 of differentiation, upon which the expression of FABP4, PLIN1 and ADIPOQ was monitored. Results in A-D are based on four biological/independent experiments. Expression of genes was normalized to the reference gene 18 s. Results were analyzed using t-test and presented in fold change ± SD relative to negative control of a corresponding time point (Neg C). ***P < 0.005, **P < 0.01, *P < 0.05.