Table 2.
Sperm motility parameters in F1-F4 generations.
| (n = 12) | Total motility (%) ± SEM | Progressive motility (%) ± SEM | VAP (µm/s) ± SEM | VSL (µm/s) ± SEM | VCL (µm/s) ± SEM | ALH (µm/s) ± SEM | BCF (Hz) ± SEM | STR (%) ± SEM | LIN (%) ± SEM |
|---|---|---|---|---|---|---|---|---|---|
| F1 | |||||||||
| CTRL | 78a ± 6 | 67a ± 5 | 326.7 ± 18 | 243.6 ± 16 | 504.9 ± 23 | 20.1 ± 0.5 | 15.9 ± 0.9 | 67a ± 1 | 46a ± 1 |
| POPs | 64b ± 6 | 53b ± 5 | 282.9 ± 18 | 204.6 ± 16 | 454.6 ± 23 | 19.5 ± 0.5 | 17.7 ± 0.9 | 65b ± 1 | 42b ± 1 |
| FA | 82a ± 6 | 70a ± 5 | 316.0 ± 18 | 228.8 ± 16 | 503.3 ± 23 | 20.8 ± 0.5 | 17.2 ± 0.9 | 65b ± 1 | 42b ± 1 |
| POPsFA | 72b ± 6 | 61b ± 5 | 308.4 ± 18 | 227.4 ± 16 | 485.0 ± 23 | 20.0 ± 0.5 | 16.9 ± 0.9 | 66a ± 1 | 45a ± 1 |
| p value | POPs (p = 0.04) | POPs (p = 0.04) | POPs (p = 0.17) | POPs (p = 0.20) | POPs (p = 0.14) | POPs (p = 0.14) | POPs (p = 0.39) | POPs (p = 0.69) | POPs (p = 0.44) |
| FA (p = 0.32) | FA (p = 0.31) | FA (p = 0.69) | FA (p = 0.79) | FA (p = 0.53) | FA (p = 0.20) | FA (p = 0.77) | FA (p = 0.69) | FA (p = 0.87) | |
| POPs*FA (p = 0.74) | POPs*FA (p = 0.63) | POPs*FA (p = 0.33) | POPs*FA (p = 0.23) | POPs*FA (p = 0.49) | POPs*FA (p = 0.80) | POPs*FA (p = 0.26) | POPs*FA (p = 0.02) | POPs*FA (p = 0.01) | |
| F2 | |||||||||
| CTRL | 52 ± 7 | 43 ± 6 | 212.5 ± 9 | 159.3 ± 8 | 319.6 ± 12 | 13.7 ± 0 | 14.9 ± 0.9 | 68a ± 1 | 47a ± 2 |
| POPs | 42 ± 8 | 32 ± 7 | 207.7 ± 11 | 164.4 ± 10 | 299.1 ± 13 | 12.5 ± 1 | 14.8 ± 1.0 | 70a ± 1 | 50a ± 2 |
| FA | 56 ± 7 | 42 ± 6 | 207.7 ± 9 | 151.0 ± 8 | 307.0 ± 12 | 13.3 ± 0 | 14.6 ± 0.9 | 66b ± 1 | 44b ± 2 |
| POPsFA | 53 ± 8 | 41 ± 6 | 189.1 ± 10 | 139.9 ± 9 | 286.7 ± 12 | 12.6 ± 1 | 15.7 ± 0.9 | 66b ± 1 | 45b ± 2 |
| p value | POPs (p = 0.40) | POPs (p = 0.37) | POPs (p = 0.24) | POPs (p = 0.72) | POPs (p = 0.10) | POPs (p = 0.09) | POPs (p = 0.60) | POPs (p = 0.36) | POPs (p = 0.26) |
| FA (p = 0.34) | FA (p = 0.50) | FA (p = 0.24) | FA (p = 0.06) | FA (p = 0.31) | FA (p = 0.80) | FA (p = 0.77) | FA (p = 0.02) | FA (p = 0.03) | |
| POPs*FA (p = 0.69) | POPs*FA (p = 0.44) | POPs*FA (p = 0.45) | POPs*FA (p = 0.36) | POPs*FA (p = 0.99) | POPs*FA (p = 0.67) | POPs*FA (p = 0.53) | POPs*FA (p = 0.44) | POPs*FA (p = 0.58) | |
| F3 | |||||||||
| CTRL | 48 ± 7 | 37 ± 5 | 241.2 ± 10 | 173.7 ± 8 | 398.2 ± 17 | 18.6 ± 7 | 19.4 ± 0.7 | 64a ± 1 | 41 ± 1 |
| POPs | 46 ± 7 | 34 ± 6 | 227.5 ± 10 | 151.0 ± 8 | 370.3 ± 17 | 18.0 ± 7 | 20.3 ± 0.7 | 61b ± 1 | 40 ± 1 |
| FA | 44 ± 7 | 34 ± 6 | 237.7 ± 10 | 161.6 ± 8 | 387.8 ± 17 | 32.4 ± 7 | 20.1 ± 0.7 | 62a ± 1 | 41 ± 1 |
| POPsFA | 52 ± 7 | 39 ± 6 | 242.2 ± 10 | 163.7 ± 8 | 397.5 ± 17 | 18.1 ± 7 | 20.4 ± 0.7 | 61b ± 1 | 40 ± 1 |
| p value | POPs (p = 0.66) | POPs (p = 0.79) | POPs (p = 0.65) | POPs (p = 0.21) | POPs (p = 0.58) | POPs (p = 0.29) | POPs (p = 0.41) | POPs (p = 0.02) | POPs (p = 0.21) |
| FA (p = 0.87) | FA (p = 0.88) | FA (p = 0.59) | FA (p = 0.96) | FA (p = 0.62) | FA (p = 0.33) | FA (p = 0.56) | FA (p = 0.80) | FA (p = 0.87) | |
| POPs*FA (p = 0.48) | POPs*FA (p = 0.43) | POPs*FA (p = 0.38) | POPs*FA (p = 0.13) | POPs*FA (p = 0.26) | POPs*FA (p = 0.33) | POPs*FA (p = 0.68) | POPs*FA (p = 0.26) | POPs*FA (p = 0.81) | |
| F4 | |||||||||
| CTRL | 56 ± 6 | 43 ± 5 | 221.0 ± 21 | 146.6a ± 20 | 349.0a ± 23 | 16.8 ± 1 | 18.9 ± 0.9 | 64 ± 1 | 43 ± 1 |
| POPs | 57 ± 6 | 45 ± 5 | 231.6 ± 21 | 167.1a ± 20 | 350.2a ± 23 | 15.7 ± 1 | 18.5 ± 0.9 | 66 ± 1 | 46 ± 1 |
| FA | 65 ± 6 | 52 ± 5 | 250.9 ± 21 | 184.7b ± 20 | 385.6b ± 23 | 16.9 ± 1 | 17.4 ± 0.9 | 66 ± 1 | 45 ± 1 |
| POPsFA | 58 ± 6 | 45 ± 5 | 279.9 ± 21 | 219.3b ± 21 | 409.3b ± 23 | 16.1 ± 1 | 17.5 ± 0.9 | 68 ± 1 | 47 ± 1 |
| p value | POPs (p = 0.56) | POPs (p = 0.62) | POPs (p = 0.34) | POPs (p = 0.17) | POPs (p = 0.58) | POPs (p = 0.10) | POPs (p = 0.87) | POPs (p = 0.14) | POPs (p = 0.14) |
| FA (p = 0.44) | FA (p = 0.38) | FA (p = 0.07) | FA (p = 0.03) | FA (p = 0.05) | FA (p = 0.62) | FA (p = 0.16) | FA (p = 0.08) | FA (p = 0.31) | |
| POPs*FA (p = 0.45) | POPs*FA (p = 0.35) | POPs*FA (p = 0.65) | POPs*FA (p = 0.72) | POPs*FA (p = 0.62) | POPs*FA (p = 0.83) | POPs*FA (p = 0.76) | POPs*FA (p = 0.91) | POPs*FA (p = 0.69) | |
(VAP: Average path velocity; VSL: Linear velocity; VCL: Curvilinear velocity; ALH: mean amplitude of lateral head displacement; BCF: frequency of head displacement; STR: straightness coefficient; LIN: linearity coefficient). SEM = standard error of the mean.