Fig. 1.

Patient with loss of PI3Kγ and T lymphocytic infiltration of lung and gut. a Chest computed tomography scan with diffuse pulmonary nodular and patchy infiltrates. b Hematoxylin and eosin staining of histological sections of a lung biopsy with lymphocytic infiltrates (top left) and regions of organizing pneumonia (top right) and gut biopsy with lymphocytic infiltrates (bottom left) and staining for CD3 (bottom right). c Pedigree with PIK3CG alleles inherited by patient A.1. d Chromatograms obtained by Sanger sequencing of PIK3CG genomic DNA from patient A.1. e Immunoblotting of p110γ, p101, and β-tubulin in T cell blasts from an unrelated healthy control, patient A.1, and parents