Fig. 4.

BDR proteins promote 3′ pausing and gene expression in a specific genomic context. a Pol II ChIP-seq coverage across expressed genes in Arabidopsis seedlings. 3′ pausing indicated by red arrows. b Calculation of a 3′ pausing index. c 3′ pausing indices for nine groups of genes defined by increasing mRNA expression levels in wild type. 3′ pausing is reduced in the bdr1,2,3 mutant, particularly at highly expressed genes. The centerline of boxplots is the median. The bounds of the box are the first and third quartiles (Q1 and Q3). Whiskers represent data range but are bounded to 1.5-fold the interquartile range (Q3–Q1); points outside this range are represented individually