Figure 6.

Analysis of interphase (G₁/S), stained and unstained, living HEK293 cells. (a) Bright-field, fluorescence and MCARS (CH₂, CH₃, merge) images of selected cells. MCARS images were reconstructed at 2850 cm⁻¹ (CH₂ symmetric stretching) and 2930 cm⁻¹ (CH₃ symmetric stretching). Scale bar, 5 µm. (b) Standard deviation of the vibrationally resonant CARS signal between 2500 and 3200 cm⁻¹, computed over the whole analysis window of each cell. Spectra are plotted vertically in the same order as corresponding cells. CH₂ and CH₃ channels are highlighted.