Table 1.
Evaluation of the SAXS scattering data of the TeLPOR apo and holoprotein
apoprotein | holoprotein | |
---|---|---|
I(0) (cm−1) | 0.013785 ± 0.00005 | 0.109217 ± 0.00037 |
M from I(0)a (Da) [dimer content (%)b] |
37,096 ± 131 [0] |
52,108 ± 176 [32.4 ± 0.3] |
Vp (Å3) | 65,730 | 96,680 |
M from Vp (Da) [dimer content (%)] |
38,649 [1.7] |
55,895 [42.0] |
Rg (Å) from Guinier | 23.0 ± 0.015 | 31.6 ± 0.013 |
Rg (Å), reciprocal space/real spacec | 22.9/22.9 ± 0.006 | 32.3/32.5 ± 0.006 |
Dmax (Å)c | 80.5 | 105.4 |
M from chemical composition | 38,014 | 39,370 |
M from ab initio modelling (Da) DAMMIF/DAMMIN/GASBORPd |
35,910/31,110/29,498 | 58,153e |
adetermined as described in Supplementary Table 2; bassuming the presence of only monomers and dimers, determined as described in Supplementary Table 2; cderived by using GNOM; ddetermined by dividing the filtered volume of the DAMAVER generated averaged and filtered model by a factor of two edetermined from the GASBORMX model of the dimer as described in d