Figure 1.

SMG promotes signaling in unstimulated JAWS II DC. Unstimulated JAWS II DC (2 × 10⁵/ml) were cultured in Static (light gray) or SMG (dark gray) conditions in media for 48 h or 72 h. (a) DC were collected after 48 h of culture, permeabilized and then stained with antibodies for pSTAT-5, pERK1/2, p-mTOR and activated caspase-3 and their corresponding isotype controls (open histogram/broken line). GM-CSFR detection was accomplished with antibody in non-permeabilized cells. (b) Graph represents the mean fluorescence intensity (MFI) of each of the molecules examined in (a) for Static (light gray bars) and SMG DC (black bars). One representative experiment of two independent experiments with similar results is shown. (c) Following 72 h of culture in Static (light gray bar) or SMG (black bar) conditions, the cells were enumerated by trypan blue exclusion. Bar graph represents the means of independent cultures (n = 16) + SD of all samples. In (b), *p-value ≤ 0.05 comparing the intracellular and surface expression of molecules by SMG and Static DC. In (c), *p-value ≤ 0.05 comparing the counts of Static and SMG DC.