Figure 6.

JAWS II DC can activate T cells within SMG but demonstrate a reduced capacity to capture and/or present antigens in long-term cultures. JAWS II DC (2 × 10⁵/ml) were cultured in Static (white and light gray bars) or SMG (dark gray and black bars) conditions for 72 hours. (a) Afterward, Static and SMG cultured JAWS II DC were harvested, washed and incubated with OT-II CD4⁺ TCH and OVA323-339 for an additional 24 hours in either Static or SMG conditions (10 ml total volume). (b) Harvested Static and SMG cultured JAWS II DC were refreshed with media and incubated with OT-II CD4⁺ TCH and OVA protein for an additional 24 hours in either Static or SMG conditions as in (a). Culture supernatants were collected and assessed for IL-2 production by ELISA. For (a,b), data represents the mean + SD of n = 10 and n = 6, respectively, of two independent experiments. In (a), *p-value ≤ 0.05 comparing the activation of T cell IL-2 production by SMG and Static DC in Static conditions as well as SMG and Static DC in SMG conditions. In (b), *p-value ≤ 0.05 comparing the activation of T cell IL-2 production by Static and SMG DC in SMG conditions.