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. 2019 Sep 25;9:13905. doi: 10.1038/s41598-019-50307-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Transient MMC treatment decreases cell migration and TGFβ1 mediated collagen deposition by human corneal fibroblasts (HCFs). (A) As indicated in the schematic, fibroblasts were treated with MMC for 3 hours, washed, and re-fed with media lacking MMC and tracked overnight. Two different media types were used for these studies: HCF media containing 10% FCS, and HCLE defined media lacking serum. The following day, live cell imaging was performed. Results show that while HCFs migrated faster in media containing serum, MMC decreased HCF migration rates significantly in both types of media. (B) As indicated in the schematic, control and HCFs were treated with MMC for 3 hours, washed, and re-fed with HCF media lacking MMC, media supplemented with vitamin C, and media with both vitamin C and TGFβ1. Results show that while vitamin C and TGFβ1 enhance collagen deposition in control cells, treating cells with MMC prevents TGFβ1 from enhancing collagen deposition by HCFs.