Fig. 6.

Overexpression of tankyrase promotes tumor growth and predicts poor clinical outcome. a Inducible expression of TNKS1 leads to AMPK inactivation. LKR13 cells stably expressing inducible TNKS1 were treated without or with doxycycline (dox:100 ng/ml) for the indicated hours, or with XAV939/G007-LK (5 μM) for 12 h followed by western blotting. b, c Inducible expression of TNKS1 promotes cell proliferation (b) and colony formation (c). LKR13 cells stably expressing inducible TNKS1 were treated without or with doxycycline (dox:100 ng/ml), or treated in combination with doxycycline plus XAV939/G007-LK, Cell proliferation and colony formation were measured as described above; data are presented as means ± SD (n = 3 independent experiments). d, e Inducible expression of TNKS1 promotes tumor growth. LKR13-inducible-TNKS1 cells (0.5 × 10⁶ cells) were implanted by injection. Tumor volume (d) and weight (e) of mice from different treatment groups; data are presented as means ± SD (n = 8 mice). f Quantification and two representative cases of immunohistochemical staining for the correlation between p-AMPK and TNKS1/2 using human lung carcinoma tumor tissue microarray analysis. Statistical significance was determined by Chi-square test. Bar, 50 μm. g, h Kaplan–Meier overall-survival analysis of TNKS1 (g) and p-AMPK (h) levels in lung adenocarcinoma and lung squamous cell carcinoma patients detected by IHC staining (n = 91 and 75 tissues, respectively, log rank test). Statistical significance was determined by a two-tailed, unpaired Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001