Skip to main content
. 2019 Sep 19;10:603. doi: 10.3389/fendo.2019.00603

Figure 2.

Figure 2

Inhibition of TLR4 protects tubular cell injury by suppressing GSDMD-related pyroptosis in db/db mice. (A) Kidney sections stained with HE in db/m (a), db/db (b), and db/db mice treated with TLR4 inhibitor (TAK-242, 3 mg/kg) for 7 days (c); (d–f): IHC staining of GSDMD in renal tissues (magnification ×200); (g–i): representative renal cortical sections of IL-18 IHC staining (magnification ×200); (j–l): representative images of TUNEL staining (arrow show) (magnification ×400). (B) Body weight changes in different groups. (C) Blood glucose levels. (D) Urinary NAG levels. (E) Tubular injury scores. (F) Western blotting and densitometric analysis of TLR4 (F1) and caspase-1 p20 (F2) expressions in kidney tissues of db/m, db/db, and db/db mice treated with TAK-242. (G) WB and densitometric analysis (G1) of GSDMD-NT in kidney tissues in different groups. (H) IL-1β level in kidney homogenate was measured by ELISA. Each assay was representative of three independent experiments. Data were expressed as means ±SEM. (*P < 0.05 compared with db/m group, **P < 0.05 compared with db/db group).