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. 2019 Sep 19;10:603. doi: 10.3389/fendo.2019.00603

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Copyright © 2019 Wang, Zhu, Yuan, Wen, Liu, Wang, Qu, Li, Liu, Sun and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR4/ NF-κB and GSDMD-NT expressions were increased in HK-2 cells under HG condition. (A) WB and densitometric analysis of TLR4 in HK-2 cells exposed to different concentrations of D-glucose. (B) Protein expression of GSDMD-NT exposed to different concentrations of D-glucose. The protein level of TLR4 (A1) and GSDMD-NT(B1) in HK-2 cells exposured to D-glucose (5.5, 17.5, 30, and 45 mM) for 24 h. Each assay was representative of three independent experiments. Data were expressed as means ±SEM. (*P < 0.05 vs. control; **P < 0.05 vs. 17.5Glu group; ***P < 0.05 vs. 30Glu group).