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. 2019 Sep 19;10:603. doi: 10.3389/fendo.2019.00603

Figure 4.

Figure 4

Inhibition of TLR4/NF-κB signaling decreased the expression of caspase-1 and GSDMD in HK-2 cells under HG condition. (A) HK-2 cells were treated with TLR4 inhibitor (TAK242, 1 μM) for 2 h prior to HG (30 mM) treatment and with NF-κB blocker (parthenolide, 5 μM) and HG (30 mM) for 2 h, phospho-NF-κB p65 increased in 30 mM HG for 2 h compared to the control, while TAK-242 and parthenolide alleviated HG-induced NF-κB p65 phosphorylation. (B–D) HK-2 cells were treated with TLR4 inhibitor (TAK-242, 1 μM) for 2 h prior to HG (30 mM) treatment for 24 h or with NF-κB blocker (parthenolide, 5 μM) and HG (30 mM) for 24 h, and samples were collected for Western blot analysis to detect caspase-1 (B1), GSDMD(B2), caspase-1 p20 (C1) and GSDMD-NT(D1) expressions. (E) IL-1β level in supernatant of HK-2 cells measured by ELISA was increased in 30 mM glucose group and reversed both in 30GLU+ TAK242 and 30GLU+ parthenolide groups. Each assay was representative of three independent experiments. Data were expressed as means ±SEM. (*P < 0.05 vs. control group; **P < 0.05 vs. 30Glu group).