Figure 5.

Downstream receptor for advanced glycation end-products (RAGE) and nuclear factor (NF)-κB pathway were inhibited after treatment of Nec-1 and melatonin. NF-κB pathway was detected by immunoblotting in the cortex (A–E) and hippocampus CA1 (F–J). Relative downstream inflammatory factors were detected by immunoblotting in the cortex (K–N) and hippocampus CA1 (O–R). All experiments were performed in triplicate by one way ANOVA plus Tukey’s test. **P < 0.01 and ***P < 0.001 vs. sham group. ^#P < 0.05 and ^##P < 0.01 vs. CCI group.