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. 2019 Sep 19;12:222. doi: 10.3389/fnmol.2019.00222

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Copyright © 2019 Bao, Fan, Zhao, Xu, Liu, Chao, Liu, You, Liu, Wang and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AAV-shA20 restored inflammatory responses. (A,B) Interleukin-6 (IL-6), interleukin-1 (IL-1β) and NLRP3 were detected in cortex tissues and hippocampal CA1 tissues by immunoblotting, respectively. The results including cortex (C–E) and hippocampus CA1 (F–H) were analyzed by statistical. (I) Representative images of double IL-1β (red) and Iba-1 (green) positive cells; NLRP3 (red) and Iba-1 (green); IL-6 (red) and GFAP (green) in the hippocampus CA1 location, as determined by immunofluorescence staining. Nuclei were stained with DAPI (blue). Data were measured by one way ANOVA plus Tukey’s test. *P < 0.05 and **P < 0.01 vs. CCI+AAV-shCtrl group. ^#P < 0.05 and ^##P < 0.01 vs. CCI+AAV-shCtrl+Nec-1 or CCI+AAV-shCtrl+melatonin group, respectively.