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. 2019 Sep 25;9:13848. doi: 10.1038/s41598-019-50124-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component analysis (PCA) plot of RNA expression. In both (a) mRNA and (b) miRNA, PC1 explained around 20% of the total variation and PC2 explained approximately 10%. PC1 closely reflects the earliest phylogenetic split among these eight cichlid fishes (as depicted with the black lines) and separated cichlid groups from the Neotropics (on the left) and Africa (to the right) into distinct lineages.