Table 1.
Plasmids and bacterial strains used in this study.
| Name | Characteristics | Reference |
|---|---|---|
| Plasmids | ||
| pNZ123 | E. coli, L. lactis and S. thermophilus shuttle vector | 31 |
| pNZ123:metAP | pNZ123 with metAP gene cloned in XbaI site | This study |
| pNZ123:metAPH206Q | pNZ123 with metAPH206Q gene cloned in XbaI site | This study |
| pNZ123:metAPSmut | pNZ123 with metAP of S. mutans HER1503 gene cloned in XbaI site | This study |
| Bacterial strains | ||
| S. thermophilus SMQ-301 | 36 | |
| S. thermophilus SMQ-301 BIM #2 | This study | |
| S. thermophilus SMQ-301 BIM #3 | This study | |
| S. thermophilus SMQ-301 BIM #5 | This study | |
| S. thermophilus SMQ-301:metAPH206Q | This study | |
| S. thermophilus SMQ-301:metAPH206Q + pNZ123: metAP | This study | |
| S. thermophilus DGCC7710 | 9 | |
| S. thermophilus DGCC7710:metAPH206Q | This study | |
| S. thermophilus DGCC7710:metAPH206Q + pNZ123: metAP | This study | |
| S. thermophilus DGCC7796 | This study | |
| S. thermophilus DGCC7796:metAPH206Q | This study | |
| S. thermophilus DGCC7796:metAPH206Q + pNZ123:metAP | This study | |
| S. thermophilus DGCC782 | This study | |
| S. thermophilus DGCC782:metAPH206Q | This study | |
| S. thermophilus DGCC782:metAPH206Q + pNZ123:metAP | This study | |
| S. mutans HER 1503 | 43 | |
| S. mutans HER 1503:metAPH206Q | This study | |
| S. mutans HER 1503:metAPH206Q + pNZ123:metAPSmut | This study | |