. 2019 Sep 25;9:13837. doi: 10.1038/s41598-019-49520-3

Table 3.

Primers and cycling conditions used in this study.

Target region/gene	Amplicon size	Primer name	Primer Sequences (5′ → 3′)	Cycling conditions						Reference
16S rRNA gene (bacterial DNA)	146 bp	q16S-univF	GTGSTGCAYGGYTGTCGTCA	95 °C	45x	95 °C	53 °C	72 °C		Maeda et al.⁹⁰
16S rRNA gene (bacterial DNA)	146 bp	q16S-univR	ACGTCRTCCMCACCTTCCTC	10 min		20 s	30 s	20 s	—	Maeda et al.⁹⁰
GAPDH (human DNA)	74 bp		TGCACCACCAACTGCTTAGC	95 °C	40x	95 °C	65 °C			Vandesompele et al.⁹¹
GAPDH (human DNA)	74 bp		GGCATGGACTGTGGTCATGAG	10 min		10 s	60 s	—	—	Vandesompele et al.⁹¹
V3/V4 16S rRNA gene (library preparation)	~460 bp	s16S_F	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-InnerTag-CCTACGGGNGGCWGCAG	95 °C	25x	95 °C	55 °C	72 °C	72 °C	Klindworth et al.⁷⁹
V3/V4 16S rRNA gene (library preparation)	~460 bp	s16S_R	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG- InnerTag-GACTACHVGGGTATCTAATCC	3 min		30 s	30 s	30 s	5 min	Klindworth et al.⁷⁹