Figure 1.

RNA-Seq analysis of MtCDF gene expression in Medicago Jester leaf tissue under vernalized short-day (VSD) and vernalized long-day (VLD) photoperiods. (A) Neighbor joining tree diagram of CDF-like proteins in Arabidopsis and Medicago using their full-length amino acid sequences. Clades of similar proteins were collapsed. See Figure S1 for a more comprehensive tree. (B–O) Derived from RNA-Seq data (Thomson et al., 2019); the mean abundance of MtCDF gene transcripts in leaf tissue at 0, 2, and 4 h after dawn in transcripts per million (TPMs) in VSD and VLD. Abundances for the three biological replicates are plotted as points, and asterisks indicate significant differential expression (Wald significance tests; α = 0.05).