Figure 2.

Overexpression of Medicago CDF genes in Arabidopsis can result in late flowering. (A) Flowering time of independent T₁ transgenic plants (n ≥ 14) derived from 11 35S:MtCDF expression vectors and Columbia wild-type Arabidopsis in LD conditions. The gray line represents the average leaves at flowering for Columbia; 11.2 ± 0.63 leaves (t.SE 0.05; n = 19). (B) Photographs of selected T₂- and T₃-generation 35S:MtCDF plants at the time of flowering. (C) Several 35S:MtCDFd1_1 and 35S:MtCDFc1 transgenic plants displayed aerial rosette phenotypes (white boxes) and poor fertility. Multiple 35S:MtCDFd1_3 and 35S:MtCDFe transgenic plants had an upright rosette leaf stature with rigid long-handle spoon-shaped leaves. Additionally, these plants were darker in color with purple abaxial surfaces but had light-colored spots on the older leaves (white boxes in the last panel) and had poor fertility. Age of the plants indicated in days. Yellow scale bars = 2 cm.