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. 2019 Sep 19;10:1148. doi: 10.3389/fpls.2019.01148

Figure 4.

Figure 4

MtCDFd1_1 overexpression in Medicago reduces MtFTa1, MtFTb1, and MtFTb2 transcript levels. (AD) Expression of MtCDFd1_1, MtFTa1, MtFTb1, and MtFTb2 in the 35S:MtCDFd1_1 Medicago R108 transgenic lines in vernalized long day (VLD), long day (LD), and vernalized short day (VSD). Data were derived from fully expanded trifoliate leaves harvested on days 14 and 15 (VLD), day 46 (LD), and day 43 (VSD) at ZT4. Gene expression levels are means ± SE of three biological replicates, normalized to PP2A. Data were presented relative to the highest value of a gene across the three growth conditions. In VLD, R108-1 was grown at the same time as lines 4.17, 13.24, 17.34, 19.30, and 1.1, while R108-2 was grown with line 2.2. In LD, R108-1 was grown at the same time as lines 17.34 and 1.1, while R108-2 with lines 4.17 and 2.2. All plants grown in VLD were T1 generation, while T2 populations were grown in LD and VSD. Asterisks indicate transgenic lines with significantly different expression from R108 [multiple pairwise comparisons adjusted for false discovery rate (FDR); α = 0.05].

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