FIG 3.

A targeted whole-cell phenotypic screen identifies six new MmpL3 inhibitors. (a) Results of a direct head-to-head comparison of percentages of growth inhibition of WT M. tuberculosis and a pooled mmpL3 mutant population treated with 163 compounds at 20 μM. Additional treatments included 0.5 μM BDQ, CLO, INH, PAS, or SQ109 or 0.03% H₂O₂. Examples of hit compounds with reduced activity in the MmpL3 mutant pool are shown in red. (b) Structures of the confirmed hit compounds from the screen, including six new compounds, HC2032, HC2099, HC2134, HC2138, HC2178, and HC2183. Previously described compounds include C215, HC2091, and SQ109.