TABLE 4.
Strains and plasmids used in this worka
| Strain or plasmid | blaOXA gene | Features | Reference or source |
|---|---|---|---|
| Strains | |||
| A. baumannii ATCC 17978 | NA | A. baumannii type strain | ATCC |
| A. baumannii ATCC 17978 + pET-RA/OXA-23 | OXA-23 | A. baumannii ATCC 17978 harboring pET-TRA-KM plasmid overexpressing OXA-23 | 10 |
| A. baumannii Ab1 | OXA-23 | A. baumannii clinical isolate from A Coruña (Spain) | 42 |
| A. baumannii ATCC 17978 + pET-RA/OXA-24/40 | OXA-24/40 | A. baumannii ATCC 17978 harboring pET-TRA-KM plasmid overexpressing OXA-24/40 | 10 |
| A. baumannii JC12/04 | OXA-24/40 | A. baumannii clinical isolate from A Coruña (Spain) | This study |
| Escherichia coli BL21 | OXA-24/40 | E. coli type strain | GE Healthcare (Chicago, IL, USA) |
| Plasmids | |||
| pET-RA-KM | Cloning vector harboring kanamycin and rifampicin resistance markers | 10 | |
| pGEX-6P-1 | Cloning vector for expressing GST fusion proteins with a PreScission protease site | GE Healthcare (Chicago, IL, USA) |
a
NA, not applicable; ATTC, American Type Culture Collection; GST, glutathione S-transferase.