FIG 1.

The pyrimidinetrione amide scaffold inhibits Mg²⁺ uptake by M. tuberculosis. (A) Magnesium dependence of the pyrimidinetrione amide MIC against M. tuberculosis in Mg²⁺-free medium with the indicated concentrations of Mg²⁺. (B) Co²⁺ dependence of the pyrimidinetrione amide MIC against M. tuberculosis in Co²⁺-free medium with the indicated concentrations of Co²⁺. (C) Fold changes in intracellular Mg²⁺ concentrations as measured by ICP-MS compared to vehicle (DMSO) control after 24 h of treatment with moxifloxacin (Mox) or compound 10 at 1- or 10-fold the MIC in 7H9 medium that contained 0.4 mM Mg²⁺. Significance between groups was determined by Tukey’s multiple-comparison test, with significance set at P values of less than 0.05. *, 0.02; ***, 0.0006; ns, not significant. The error bars indicate standard deviation.