FIG 2.

Magnesium and the pyrimidinetrione scaffold bind to the CorA transporter protein. The thermostability of recombinantly expressed CorA from M. tuberculosis was measured with no ligand (A), 0.125 mM MgCl₂ (B), and 0.125 mM MgCl₂ with or without 1.0 mM compound 10 (C). The protein was preincubated at room temperature with the indicated ligands before heating to the indicated temperatures in a thermocycler. Protein precipitates were removed by filtration, and soluble protein was analyzed by SDS-PAGE.