FIG 2.
RpoN is required for stationary-phase susceptibility to aminoglycosides combined with C4-dicarboxylates. Shown are survival data for PA14 wild-type and ΔrpoN stationary-phase cells that were incubated for 4 h with no treatment, various concentrations of an aminoglycoside, 15 mM a C4-dicarboxylate, or an aminoglycoside with a C4-dicarboxylate. The tested aminoglycoside and C4-dicarboxylate combinations were TOB+SUC (A), GEN+FUM (B), and AMK+FUM (C). Data are presented as mean log10 CFU per milliliter ± SEM for three biological replicates.