FIG 5.
Loss-of-function RpoN alleles from CF clinical isolates impair TOB+FUM killing. (A) Schematic representation of RpoN showing some mutations in the DNA binding domain that have been previously observed in CF clinical isolates. aa, amino acids. (B) Survival of PA14 wild-type and ΔrpoN stationary-phase cells carrying pMQ70, pMQ70::rpoN, or pMQ70::rpoN with clinically relevant point mutations following incubation for 4 h with or without 64 μg/ml TOB and/or 15 mM FUM. Data are presented as mean log10 CFU per milliliter ± SEM for three biological replicates.