FIG 6.
TOB+FUM specifically kills cells with RpoN function in mixed-genotype populations. (A to E) PA14-lacZ and ΔrpoN stationary-phase cells were mixed at 1:100 (A), 1:10 (B), 1:1 (C), 10:1 (D), and 100:1 (E) ratios, and survival of individual genotypes was determined prior to treatment (0 h) or following 4 h of incubation with either no treatment or TOB+FUM (64 μg/ml TOB and 15 mM FUM). Data are presented as mean log10 CFU per milliliter ± SEM for three biological replicates. (F) Data in panels A to E were used to calculate log10 PA14-lacZ/ΔrpoN ratios of the populations. TOB+FUM treatment consistently reduced the log10 PA14-lacZ/ΔrpoN ratios, indicating a decrease in PA14-lacZ cells relative to ΔrpoN cells in the population.