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. 2019 Sep 19;10:1149. doi: 10.3389/fpls.2019.01149

Figure 5.

Figure 5

Transient expression of OeRbohH in tobacco pollen tubes as YFP fusions, observed by CLSM. (A) YFP construction alone (control) showed homogeneous yellow fluorescence throughout the cytoplasm of the pollen tube. (B) YFP : OeRbohH and (C–F) OeRbohH : YFP transformants showed labeling in the plasma membrane (arrowheads) as well as in the cytoplasm without significant differences among both constructs. (D) Fluorescence was also observed at the edge of callose plugs in their proximal side (referred to the pollen grain). The majority of transformed pollen tubes showed accumulation spots (C, E, F) and abnormal phenotypes (i.e., swelling) at the tip (E–F), and their growth was significantly inhibited after 4h in vitro culture (G). Insets at pictures B, C, and D show bright field images of the pollen tubes for reference. CP, callose plug; Cy, cytoplasm. *indicates that the mean is significantly different from the control at P < 0.05. n = 100 from three independent experiments. Bars = 20 µm.