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. 2019 Sep 19;10:1149. doi: 10.3389/fpls.2019.01149

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Copyright © 2019 Jimenez-Quesada, Traverso, Potocký, Žárský and Alché

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DPI inhibits germination and pollen tube elongation. Control sample (A). When the NADPH oxidase inhibitor was added at the beginning of the process, the in vitro germination percentage was affected (B). When the inhibitor was added during the germination, the length of the pollen tube was affected (C) in both cases when compared to the control. Representative pictures from three independent experiments are shown. Quantification of the germination rate (D) and pollen tube lengths (E). Data represent means ± SEM. n = 100. *indicates that the mean is significantly different from the control at P < 0.05. Bars = 100 µm.