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. 2019 Sep 19;10:1149. doi: 10.3389/fpls.2019.01149

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Copyright © 2019 Jimenez-Quesada, Traverso, Potocký, Žárský and Alché

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) In gel NADPH oxidase activity of mature (MP), hydrated (H), and germinated pollen extracts at different times (1, 4, 8, and 12 h) fractionated by native PAGE (50 µg/lane). (B) In mature pollen, the activity revealed by NBT was challenged by the addition of sodium azide or DPI during the incubation. (C, D) Densitometry quantification of NADPH oxidase activity as in (A) and (B), respectively. *indicates that the mean from three independent experiments is significantly different from the control (mature pollen) at P < 0.05.