Figure 9.

Transfection of olive pollen tubes with OeRbohH-specific antisense oligodeoxynucleotides (ODNs). The OeRbohH sequence obtained was used to design antisense ODNs. Control samples (A, E). Transfection of olive pollen tubes with such antisense ODNs resulted in pollen tube growth inhibition (B) as well as in a reduction of the production of tip-localized O₂^•− (F) when compared with the control samples or the sense ODNs (C, G). Quantification of pollen tube lengths (D) and precipitate intensity (H). Data represent means ± SEM; n = 300. *indicates that the mean value is significantly different from the control at P < 0.05. Bars in upper panel = 100 µm. Bars in bottom panel = 10 µm.