The 7-day longitudinal experimental schedule. Scaffold implantation was done on day 0. LASCA/PA imaging was on day 0 (i.e., after implantation), 1, 3, and 7. bFGF or PBS was injected subcutaneously adjacent to each scaffold every 2 days. Scaffolds were retrieved on day 7 for H&E and CD31 staining. Each mouse received two scaffolds represented by the blue areas. bFGF, basic fibroblast growth factor; H&E, hematoxylin and eosin; LASCA, laser speckle contrast analysis; PA, photoacoustic; PBS, phosphate buffered saline. Color images are available online.