Table 5. HPLC Gradient Elution.
| time | module | event | percentage of buffer B (%) |
|---|---|---|---|
| 0.01 | system controller | start | |
| 1.00 | pumps | % B | 20 |
| 7.00 | pumps | % B | 100 |
| 8.50 | pumps | % B | 100 |
| 8.51 | pumps | % B | 20 |
| 10.0 | system controller | stop |
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