Skip to main content
. 2019 Jul 17;8(15):e012273. doi: 10.1161/JAHA.119.012273

Figure 2.

Figure 2

Sildenafil treatment improves E‐C coupling protein activation and expression in mice fed a high fat diet (HFD). Images and quantification of Western blots for phosphodiesterase 5A (PDE5A), phosphodiesterase 3A (PDE3A), phospho‐serine 282 (p282) and total cardiac myosin binding protein C (cMyBP‐C), phospho‐serine 23/24 (p23/24) and total troponin I (TnI), sarcoplasmic‐endoplasmic reticulum calcium ATPase 2A (SERCA2A), and phospho‐serine 16 (p16), and total phospholamban (PLB) for normal chow (NC) and HFD mice (A and B) followed by that for HFD and HFD‐sildenafil mice (C and D). Total protein normalized to glyceraldehyde 3‐phosphate (GAPDH). *P≤0.05, **P≤0.01, ***P≤0.001 by unpaired t test. n=3 mice per group.