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. 2019 Jul 17;8(15):e012273. doi: 10.1161/JAHA.119.012273

Figure 6.

Figure 6

Sildenafil acts through β2 adrenergic receptor (β2AR) to improve adrenergic excitation‐contraction coupling response in high fat diet (HFD) adult ventriculomyocytes (AVMs). A, Contractility, (B) calcium transient amplitude, and (C) calcium transient tau of AVMs from wildtype (WT) and β2AR knockout (β2KO) mice fed NC or HFD for 4.5 months and treated ex vivo with drugs, as indicated. n≥20 cells per group. D, Contractility, (E) calcium transient amplitude, and (F) calcium transient tau of AVMs from WT mice fed HFD for 4.5 months. Cells were pretreated with and without ICI‐118551 (ICI) or CGP‐20712 (CGP) before stimulation with isoproterenol and/or sildenafil. n≥25 cells per group. For all graphs, † P≤0.05, †† P≤0.01, ††† P≤0.001, †††† P≤0.0001 compared with basal; and *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001 by 1‐way ANOVA and Tukey post‐test.