Figure 2.

Paracrine factor release from implantable bioreactor (IBR) in vitro. Assessment of conditioned media collected from outside IBRs containing 10⁶ human mesenchymal stem cells (MSCs) over 7 days in culture showed production and release of vascular endothelial growth factor (VEGF; green), interleukin 8 (IL‐8; red), basic fibroblast growth factor (bFGF; blue), and hepatocyte growth factor (HGF; orange) as assessed by multiplex ELISA (A; log scale; n=6). A subsequent dose‐finding experiment using IBRs loaded with increasing doses of hMSCs showed dose‐dependent VEGF production up to a dose of 2.5×10⁷ MSCs (B; log scale; n=6).